Host species restriction of Middle East respiratory syndrome coronavirus through its receptor, dipeptidyl peptidase 4.
Identifieur interne : 001C37 ( Main/Exploration ); précédent : 001C36; suivant : 001C38Host species restriction of Middle East respiratory syndrome coronavirus through its receptor, dipeptidyl peptidase 4.
Auteurs : Neeltje Van Doremalen [États-Unis] ; Kerri L. Miazgowicz [États-Unis] ; Shauna Milne-Price [États-Unis] ; Trenton Bushmaker [États-Unis] ; Shelly Robertson [États-Unis] ; Dana Scott [États-Unis] ; Joerg Kinne [Émirats arabes unis] ; Jason S. Mclellan [États-Unis] ; Jiang Zhu [États-Unis] ; Vincent J. MunsterSource :
- Journal of virology [ 1098-5514 ] ; 2014.
Descripteurs français
- KwdFr :
- Animaux, Bovins, Bétail (métabolisme), Bétail (virologie), Capra (métabolisme), Capra (virologie), Cellules Vero, Chameaux (métabolisme), Chameaux (virologie), Coronavirus (métabolisme), Coronavirus (pathogénicité), Cricetinae, Dipeptidyl peptidase 4 (métabolisme), Furets (métabolisme), Furets (virologie), Humains, Liaison aux protéines, Lignée cellulaire, Lignée cellulaire tumorale, Macaca mulatta (métabolisme), Macaca mulatta (virologie), Moyen Orient, Ovis (métabolisme), Ovis (virologie), Primates (métabolisme), Primates (virologie), Récepteurs viraux (métabolisme), Réplication virale (génétique), Souris, Souris de lignée C57BL, Spécificité d'hôte, Tropisme viral, Virus respiratoires syncytiaux (métabolisme), Virus respiratoires syncytiaux (pathogénicité).
- MESH :
- génétique : Réplication virale.
- métabolisme : Bétail, Capra, Chameaux, Coronavirus, Dipeptidyl peptidase 4, Furets, Macaca mulatta, Ovis, Primates, Récepteurs viraux, Virus respiratoires syncytiaux.
- pathogénicité : Coronavirus, Virus respiratoires syncytiaux.
- virologie : Bétail, Capra, Chameaux, Furets, Macaca mulatta, Ovis, Primates.
- Animaux, Bovins, Cellules Vero, Cricetinae, Humains, Liaison aux protéines, Lignée cellulaire, Lignée cellulaire tumorale, Moyen Orient, Souris, Souris de lignée C57BL, Spécificité d'hôte, Tropisme viral.
English descriptors
- KwdEn :
- Animals, Camelus (metabolism), Camelus (virology), Cattle, Cell Line, Cell Line, Tumor, Coronavirus (metabolism), Coronavirus (pathogenicity), Cricetinae, Dipeptidyl Peptidase 4 (metabolism), Ferrets (metabolism), Ferrets (virology), Goats (metabolism), Goats (virology), Host Specificity, Humans, Livestock (metabolism), Livestock (virology), Macaca mulatta (metabolism), Macaca mulatta (virology), Mice, Mice, Inbred C57BL, Middle East, Primates (metabolism), Primates (virology), Protein Binding, Receptors, Virus (metabolism), Respiratory Syncytial Viruses (metabolism), Respiratory Syncytial Viruses (pathogenicity), Sheep (metabolism), Sheep (virology), Vero Cells, Viral Tropism, Virus Replication (genetics).
- MESH :
- chemical , metabolism : Dipeptidyl Peptidase 4, Receptors, Virus.
- geographic : Middle East.
- genetics : Virus Replication.
- metabolism : Camelus, Coronavirus, Ferrets, Goats, Livestock, Macaca mulatta, Primates, Respiratory Syncytial Viruses, Sheep.
- pathogenicity : Coronavirus, Respiratory Syncytial Viruses.
- virology : Camelus, Ferrets, Goats, Livestock, Macaca mulatta, Primates, Sheep.
- Animals, Cattle, Cell Line, Cell Line, Tumor, Cricetinae, Host Specificity, Humans, Mice, Mice, Inbred C57BL, Protein Binding, Vero Cells, Viral Tropism.
Abstract
Middle East respiratory syndrome coronavirus (MERS-CoV) emerged in 2012. Recently, the MERS-CoV receptor dipeptidyl peptidase 4 (DPP4) was identified and the specific interaction of the receptor-binding domain (RBD) of MERS-CoV spike protein and DPP4 was determined by crystallography. Animal studies identified rhesus macaques but not hamsters, ferrets, or mice to be susceptible for MERS-CoV. Here, we investigated the role of DPP4 in this observed species tropism. Cell lines of human and nonhuman primate origin were permissive of MERS-CoV, whereas hamster, ferret, or mouse cell lines were not, despite the presence of DPP4. Expression of human DPP4 in nonsusceptible BHK and ferret cells enabled MERS-CoV replication, whereas expression of hamster or ferret DPP4 did not. Modeling the binding energies of MERS-CoV spike protein RBD to DPP4 of human (susceptible) or hamster (nonsusceptible) identified five amino acid residues involved in the DPP4-RBD interaction. Expression of hamster DPP4 containing the five human DPP4 amino acids rendered BHK cells susceptible to MERS-CoV, whereas expression of human DPP4 containing the five hamster DPP4 amino acids did not. Using the same approach, the potential of MERS-CoV to utilize the DPP4s of common Middle Eastern livestock was investigated. Modeling of the DPP4 and MERS-CoV RBD interaction predicted the ability of MERS-CoV to bind the DPP4s of camel, goat, cow, and sheep. Expression of the DPP4s of these species on BHK cells supported MERS-CoV replication. This suggests, together with the abundant DPP4 presence in the respiratory tract, that these species might be able to function as a MERS-CoV intermediate reservoir.
DOI: 10.1128/JVI.00676-14
PubMed: 24899185
Affiliations:
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Miazgowicz</name><affiliation wicri:level="2"><nlm:affiliation>Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana</wicri:regionArea><placeName><region type="state">Montana</region></placeName></affiliation></author><author><name sortKey="Milne Price, Shauna" sort="Milne Price, Shauna" uniqKey="Milne Price S" first="Shauna" last="Milne-Price">Shauna Milne-Price</name><affiliation wicri:level="2"><nlm:affiliation>Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana</wicri:regionArea><placeName><region type="state">Montana</region></placeName></affiliation></author><author><name sortKey="Bushmaker, Trenton" sort="Bushmaker, Trenton" uniqKey="Bushmaker T" first="Trenton" last="Bushmaker">Trenton Bushmaker</name><affiliation wicri:level="2"><nlm:affiliation>Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana</wicri:regionArea><placeName><region type="state">Montana</region></placeName></affiliation></author><author><name sortKey="Robertson, Shelly" sort="Robertson, Shelly" uniqKey="Robertson S" first="Shelly" last="Robertson">Shelly Robertson</name><affiliation wicri:level="2"><nlm:affiliation>Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana</wicri:regionArea><placeName><region type="state">Montana</region></placeName></affiliation></author><author><name sortKey="Scott, Dana" sort="Scott, Dana" uniqKey="Scott D" first="Dana" last="Scott">Dana Scott</name><affiliation wicri:level="2"><nlm:affiliation>Rocky Mountain Veterinary Branch, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Rocky Mountain Veterinary Branch, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana</wicri:regionArea><placeName><region type="state">Montana</region></placeName></affiliation></author><author><name sortKey="Kinne, Joerg" sort="Kinne, Joerg" uniqKey="Kinne J" first="Joerg" last="Kinne">Joerg Kinne</name><affiliation wicri:level="1"><nlm:affiliation>Central Veterinary Research Laboratories, Dubai, Dubai, United Arab Emirates.</nlm:affiliation><country xml:lang="fr">Émirats arabes unis</country><wicri:regionArea>Central Veterinary Research Laboratories, Dubai, Dubai</wicri:regionArea><wicri:noRegion>Dubai</wicri:noRegion></affiliation></author><author><name sortKey="Mclellan, Jason S" sort="Mclellan, Jason S" uniqKey="Mclellan J" first="Jason S" last="Mclellan">Jason S. Mclellan</name><affiliation wicri:level="2"><nlm:affiliation>Department of Biochemistry, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biochemistry, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire</wicri:regionArea><placeName><region type="state">New Hampshire</region></placeName></affiliation></author><author><name sortKey="Zhu, Jiang" sort="Zhu, Jiang" uniqKey="Zhu J" first="Jiang" last="Zhu">Jiang Zhu</name><affiliation wicri:level="2"><nlm:affiliation>Department of Immunology and Microbial Science and Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, California, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Immunology and Microbial Science and Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, California</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Munster, Vincent J" sort="Munster, Vincent J" uniqKey="Munster V" first="Vincent J" last="Munster">Vincent J. Munster</name><affiliation><nlm:affiliation>Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA vincent.munster@nih.gov.</nlm:affiliation><wicri:noCountry code="subField">USA vincent.munster@nih.gov.</wicri:noCountry></affiliation></author></analytic><series><title level="j">Journal of virology</title><idno type="eISSN">1098-5514</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Camelus (metabolism)</term><term>Camelus (virology)</term><term>Cattle</term><term>Cell Line</term><term>Cell Line, Tumor</term><term>Coronavirus (metabolism)</term><term>Coronavirus (pathogenicity)</term><term>Cricetinae</term><term>Dipeptidyl Peptidase 4 (metabolism)</term><term>Ferrets (metabolism)</term><term>Ferrets (virology)</term><term>Goats (metabolism)</term><term>Goats (virology)</term><term>Host Specificity</term><term>Humans</term><term>Livestock (metabolism)</term><term>Livestock (virology)</term><term>Macaca mulatta (metabolism)</term><term>Macaca mulatta (virology)</term><term>Mice</term><term>Mice, Inbred C57BL</term><term>Middle East</term><term>Primates (metabolism)</term><term>Primates (virology)</term><term>Protein Binding</term><term>Receptors, Virus (metabolism)</term><term>Respiratory Syncytial Viruses (metabolism)</term><term>Respiratory Syncytial Viruses (pathogenicity)</term><term>Sheep (metabolism)</term><term>Sheep (virology)</term><term>Vero Cells</term><term>Viral Tropism</term><term>Virus Replication (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Bovins</term><term>Bétail (métabolisme)</term><term>Bétail (virologie)</term><term>Capra (métabolisme)</term><term>Capra (virologie)</term><term>Cellules Vero</term><term>Chameaux (métabolisme)</term><term>Chameaux (virologie)</term><term>Coronavirus (métabolisme)</term><term>Coronavirus (pathogénicité)</term><term>Cricetinae</term><term>Dipeptidyl peptidase 4 (métabolisme)</term><term>Furets (métabolisme)</term><term>Furets (virologie)</term><term>Humains</term><term>Liaison aux protéines</term><term>Lignée cellulaire</term><term>Lignée cellulaire tumorale</term><term>Macaca mulatta (métabolisme)</term><term>Macaca mulatta (virologie)</term><term>Moyen Orient</term><term>Ovis (métabolisme)</term><term>Ovis (virologie)</term><term>Primates (métabolisme)</term><term>Primates (virologie)</term><term>Récepteurs viraux (métabolisme)</term><term>Réplication virale (génétique)</term><term>Souris</term><term>Souris de lignée C57BL</term><term>Spécificité d'hôte</term><term>Tropisme viral</term><term>Virus respiratoires syncytiaux (métabolisme)</term><term>Virus respiratoires syncytiaux (pathogénicité)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Dipeptidyl Peptidase 4</term><term>Receptors, Virus</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Middle East</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Virus Replication</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Réplication virale</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Camelus</term><term>Coronavirus</term><term>Ferrets</term><term>Goats</term><term>Livestock</term><term>Macaca mulatta</term><term>Primates</term><term>Respiratory Syncytial Viruses</term><term>Sheep</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Bétail</term><term>Capra</term><term>Chameaux</term><term>Coronavirus</term><term>Dipeptidyl peptidase 4</term><term>Furets</term><term>Macaca mulatta</term><term>Ovis</term><term>Primates</term><term>Récepteurs viraux</term><term>Virus respiratoires syncytiaux</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Coronavirus</term><term>Respiratory Syncytial Viruses</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Coronavirus</term><term>Virus respiratoires syncytiaux</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Bétail</term><term>Capra</term><term>Chameaux</term><term>Furets</term><term>Macaca mulatta</term><term>Ovis</term><term>Primates</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Camelus</term><term>Ferrets</term><term>Goats</term><term>Livestock</term><term>Macaca mulatta</term><term>Primates</term><term>Sheep</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cattle</term><term>Cell Line</term><term>Cell Line, Tumor</term><term>Cricetinae</term><term>Host Specificity</term><term>Humans</term><term>Mice</term><term>Mice, Inbred C57BL</term><term>Protein Binding</term><term>Vero Cells</term><term>Viral Tropism</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Bovins</term><term>Cellules Vero</term><term>Cricetinae</term><term>Humains</term><term>Liaison aux protéines</term><term>Lignée cellulaire</term><term>Lignée cellulaire tumorale</term><term>Moyen Orient</term><term>Souris</term><term>Souris de lignée C57BL</term><term>Spécificité d'hôte</term><term>Tropisme viral</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Middle East respiratory syndrome coronavirus (MERS-CoV) emerged in 2012. Recently, the MERS-CoV receptor dipeptidyl peptidase 4 (DPP4) was identified and the specific interaction of the receptor-binding domain (RBD) of MERS-CoV spike protein and DPP4 was determined by crystallography. Animal studies identified rhesus macaques but not hamsters, ferrets, or mice to be susceptible for MERS-CoV. Here, we investigated the role of DPP4 in this observed species tropism. Cell lines of human and nonhuman primate origin were permissive of MERS-CoV, whereas hamster, ferret, or mouse cell lines were not, despite the presence of DPP4. Expression of human DPP4 in nonsusceptible BHK and ferret cells enabled MERS-CoV replication, whereas expression of hamster or ferret DPP4 did not. Modeling the binding energies of MERS-CoV spike protein RBD to DPP4 of human (susceptible) or hamster (nonsusceptible) identified five amino acid residues involved in the DPP4-RBD interaction. Expression of hamster DPP4 containing the five human DPP4 amino acids rendered BHK cells susceptible to MERS-CoV, whereas expression of human DPP4 containing the five hamster DPP4 amino acids did not. Using the same approach, the potential of MERS-CoV to utilize the DPP4s of common Middle Eastern livestock was investigated. Modeling of the DPP4 and MERS-CoV RBD interaction predicted the ability of MERS-CoV to bind the DPP4s of camel, goat, cow, and sheep. Expression of the DPP4s of these species on BHK cells supported MERS-CoV replication. This suggests, together with the abundant DPP4 presence in the respiratory tract, that these species might be able to function as a MERS-CoV intermediate reservoir.</div></front></TEI><affiliations><list><country><li>Émirats arabes unis</li><li>États-Unis</li></country><region><li>Californie</li><li>Montana</li><li>New Hampshire</li></region></list><tree><noCountry><name sortKey="Munster, Vincent J" sort="Munster, Vincent J" uniqKey="Munster V" first="Vincent J" last="Munster">Vincent J. Munster</name></noCountry><country name="États-Unis"><region name="Montana"><name sortKey="Van Doremalen, Neeltje" sort="Van Doremalen, Neeltje" uniqKey="Van Doremalen N" first="Neeltje" last="Van Doremalen">Neeltje Van Doremalen</name></region><name sortKey="Bushmaker, Trenton" sort="Bushmaker, Trenton" uniqKey="Bushmaker T" first="Trenton" last="Bushmaker">Trenton Bushmaker</name><name sortKey="Mclellan, Jason S" sort="Mclellan, Jason S" uniqKey="Mclellan J" first="Jason S" last="Mclellan">Jason S. Mclellan</name><name sortKey="Miazgowicz, Kerri L" sort="Miazgowicz, Kerri L" uniqKey="Miazgowicz K" first="Kerri L" last="Miazgowicz">Kerri L. Miazgowicz</name><name sortKey="Milne Price, Shauna" sort="Milne Price, Shauna" uniqKey="Milne Price S" first="Shauna" last="Milne-Price">Shauna Milne-Price</name><name sortKey="Robertson, Shelly" sort="Robertson, Shelly" uniqKey="Robertson S" first="Shelly" last="Robertson">Shelly Robertson</name><name sortKey="Scott, Dana" sort="Scott, Dana" uniqKey="Scott D" first="Dana" last="Scott">Dana Scott</name><name sortKey="Zhu, Jiang" sort="Zhu, Jiang" uniqKey="Zhu J" first="Jiang" last="Zhu">Jiang Zhu</name></country><country name="Émirats arabes unis"><noRegion><name sortKey="Kinne, Joerg" sort="Kinne, Joerg" uniqKey="Kinne J" first="Joerg" last="Kinne">Joerg Kinne</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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